Detailed view for Tb927.10.7940

Basic information

TDR Targets ID: 14485
Trypanosoma brucei, methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9797 | Length (AA): 370 | MW (Da): 40896 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01728   FtsJ-like methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0080009   GO:mRNA methylation  

GO:0004483   mRNA (nucleoside-2'-O-)-methyltransferase activity  
GO:0008168   methyltransferase activity  
GO:0003676   nucleic acid binding  
GO:0032259   methylation  
GO:0006370   mRNA capping  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129738)

Species Accession Gene Product
Brugia malayi Bm1_31430   FtsJ-like methyltransferase family protein
Caenorhabditis elegans CELE_Y53F4B.13   Protein Y53F4B.13, isoform D
Dictyostelium discoideum DDB_G0271428   hypothetical protein
Dictyostelium discoideum DDB_G0279593   D111/G-patch domain-containing protein
Drosophila melanogaster Dmel_CG6379   CG6379 gene product from transcript CG6379-RB
Echinococcus granulosus EgrG_000744100   cap specific mRNA
Echinococcus multilocularis EmuJ_000744100   cap specific mRNA
Giardia lamblia GL50803_103058   Methyltransferase, putative
Giardia lamblia GL50803_100887   Methyltransferase, putative
Homo sapiens ENSG00000137200   cap methyltransferase 1
Leishmania braziliensis LbrM.35.7020   methyltransferase, putative,ensangp00000010174-like protein
Leishmania donovani LdBPK_366970.1   methyltransferase, putative
Leishmania infantum LinJ.36.6970   methyltransferase, putative,ensangp00000010174-like protein
Leishmania major LmjF.36.6660   methyltransferase, putative,ensangp00000010174-like protein
Leishmania mexicana LmxM.36.6660   methyltransferase, putative,ensangp00000010174-like protein
Loa Loa (eye worm) LOAG_08398   hypothetical protein
Mus musculus 74157   cap methyltransferase 1
Schistosoma japonicum Sjp_0046810   ko:K03143 transcription initiation factor TFIIH subunit H3, putative
Schistosoma japonicum Sjp_0046820   similar to Uncharacterized protein KIAA0082 homolog, putative
Schistosoma mansoni Smp_182200   hypothetical protein
Schistosoma mansoni Smp_186690   hypothetical protein
Schistosoma mansoni Smp_046180   hypothetical protein
Schistosoma mansoni Smp_122770   hypothetical protein
Schistosoma mansoni Smp_122730   hypothetical protein
Schistosoma mansoni Smp_108950   hypothetical protein
Schistosoma mansoni Smp_192160   hypothetical protein
Schistosoma mansoni Smp_117450   hypothetical protein
Schmidtea mediterranea mk4.002152.02   Cap-specific mRNA
Schmidtea mediterranea mk4.003505.02   Cap-specific mRNA
Trypanosoma brucei gambiense Tbg972.10.9720   methyltransferase, putative
Trypanosoma brucei Tb927.10.7940   methyltransferase, putative
Trypanosoma congolense TcIL3000_10_6820   methyltransferase, putative
Trypanosoma cruzi TcCLB.506247.320   methyltransferase, putative
Trichomonas vaginalis TVAG_116350   conserved hypothetical protein

Essentiality

Tb927.10.7940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7940 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7940 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7940 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.7940 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y53F4B.13 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.7940 (Trypanosoma brucei), methyltransferase, putative
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